Investigation of Apoptotic Effects of Aspirin Use on Human Neuroblastoma SK-N-Mc Cell Line

Abstract

Objective:Apoptosis, which is a programmed cell death, is an event in which cells destroy themselves without  inflammation,  as  well  as  requiring  energy  and  homeostasis  is  preserved. In  cases  where apoptosis  slows  down,  autoimmune  diseases  and  cancer  occur.  Aspirin  is  a  salicylic  ester  of acetic acid  and  has  analgesic,  anti-inflammatory,  antipyretic  and  antithrombotic  effects.  In  this  study,  we investigated the effects of aspirin on apoptosis in human neuroblastoma SK-N-MC cell line in vitro.Methods:In our study, trypan blue viability test (Tyripan Blue Exclusion) method was used for aspirin doses to be given to the cells. The apoptotic effect of aspirin in the SK-N-MC cell line was determined by flow cytometry imaging using APC Anexin V antibody.Results: The   aspirin   was   incubated   in   SK-N-MC   cells   at   concentrations   of   10-100μM,   and measurements  at  24  and  48  hours  were  recorded. According  to  trypan  blue  results,  cell  density decreased  proportionally  with  increasing  aspirin  concentration  compared  to  the  control  group. The cytotoxic dose was found to be between 50-80 μM at the 24th hour and 70 μM at the 48th hour.Anexin V  flow  cytometry  measurement  results  showed  that  60  and  70  μM  aspirin  given  to  SK-N-MC  cells increased early apoptosis compared to control group.Conclusion:The results of our study showed that aspirin inducedapoptosis in SK-N-MC cells in vitrodepending on dose and time.