In vitro cytotoxic effects and quality control studies on black cumin seed capsules and oils in turkey market

Abstract

ObjectiveBlack cumin seed is a species of Nigella sativa from the Ranunculacea family. thymoquinone, which is most  important  components  of  black  cumin  seed  oil,  is  preferred  as  a  traditional  source  of  healing considering its potential medicinal properties. MethodsBlack cumin seed oil and soft capsules with different formulations produced by different companies in our country were purchased, quality control tests, invitro cytotoxicity tests and antioxidant tests were performed on these oils and capsules. ResultsThe highest amount of thymoquinone was seen in black cumin seed fat 2 (O2) (2.9728 mg / ml), while the least amount of thymoquinone was in soft capsule 1 (SC1) group (0.8917 mg / ml). According to the cell viability results obtained by MTT test, O2 experimental group had the highest cytotoxic effect with 90.21%, while SC1 experimental group had the lowest cytotoxicity with 94.98%. When the antioxidant values were examined, the highest value (913,363 μg GAE / mg) was found in fat 1 (O1) and the lowest value (180,181 μg GAE / mg) was obtained in SC1.ConclusionOur study suggests that the cytotoxic effect of black cumin seed can be a promising aspect, especiallyin the development of new treatment methods and anti-cancer drugs