Vitamin D Receptors Gene Polymorphisms among Sudanese Patients with Intracerebral Hemorrhage, Khartoum State 2022.

Abstract

Background:

Cerebral bleed, intraparenchymal bleed, and hemorrhagic stroke are all terms for intracerebral hemorrhage. It accounts for about 10% of all strokes. The prevalence of stroke among Sudanese people with diabetes was 2.5% in that country. A neuroprotective hormone called vitamin D receptor (VDR), also called the calcitriol receptor, controls the differentiation of monocyte-derived macrophage from monocytes, which aids in the removal of hematomas and neurological recovery following intracerebral hemorrhage (ICH).

The objectives of this study are to estimate the prevalence of Taq I gene polymorphism (rs731236) in Sudanese patients with intracerebral hemorrhage, estimate the prevalence of Taq I gene polymorphism (rs731236) in Sudanese people without intracerebral hemorrhage, and compare the two participant groups to determine whether there are any differences.

Materials and methods:

This study was cross sectional hospital-based study, conducted at the research laboratory of the national center of neurological sciences (NCNS), Khartoum, Sudan. Patients attended to the center and diagnosed with intracerebral hemorrhage were included. DNA extraction was carried out from blood of all patients and controls, PCR for Vitamin D receptor gene was done and thus Sanger sequencing.

Results:

The PCR results revealed242 bp VDR gene presence in both cases and controls. Sequencingoutcomes showed the detection of VDR gene (T>C) rs731236 and (G>T) rs7975232 polymorphisms.

Conclusion:

VDR gene polymorphism TaqI (T>C rs731236) was found in 86%of cases suggesting this mutation might be a risk factor for Intracerebral hemorrhage.